Analysis Note

Positive ControlNIH3T3 cells, Human stomach tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (0.5 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.

Recognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.

This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.

Immunogen

A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)